Publication Details

In traumatic brain injury biomechanics, macro-scale biomechanical events need to be correlated with micro-scale neuropathological changes and improved quantitation of microscopic axonal injury is an essential component of lesion evaluation.

Objectives

To develop a novel technique for automatically identifying injured amyloid precursor protein (APP) immunopositive axons and aggregating these observations over a macroscopic brain dissection.

Methods

A color deconvolution method was adapted into Matlab to identify clusters of pixels with colors typical of APP positive tissue from large scale brain dissection.

Results

The methodology is demonstrated in the brain of a sheep subjected to a controlled cortical indentation

Conclusion

The technique will be of interest to pathologists and bioengineers seeking to quantitate brain injury over macro-scales.