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|Title||Semiquantitation of Axonal Injury in Traumatically Damaged Brains Using Color Deconvolution|
|Authors||Dutschke JK, Finney JW, Manavis J, Anderson RWG|
In traumatic brain injury biomechanics, macro-scale biomechanical events need to be correlated with micro-scale neuropathological changes and improved quantitation of microscopic axonal injury is an essential component of lesion evaluation.
To develop a novel technique for automatically identifying injured amyloid precursor protein (APP) immunopositive axons and aggregating these observations over a macroscopic brain dissection.
A color deconvolution method was adapted into Matlab to identify clusters of pixels with colors typical of APP positive tissue from large scale brain dissection.
The methodology is demonstrated in the brain of a sheep subjected to a controlled cortical indentation
The technique will be of interest to pathologists and bioengineers seeking to quantitate brain injury over macro-scales.
|Journal Title||Applied Immunohistochemistry & Molecular Morphology|
|Journal Volume (Issue)||25(4)|
|Dutschke JK, Finney JW, Manavis J, Anderson RWG (2017) 'Semiquantitation of Axonal Injury in Traumatically Damaged Brains Using Color Deconvolution', Applied Immunohistochemistry & Molecular Morphology, 25(4), pp 277-281.|